Artigos científicos


  • Puigdemont A, D'Andreano S, Ramió-Lluch L, Cuscó A, Francino O, Brazis P. Effect of an anti-inflammatory pomegranate otic treatment on the clinical evolution and microbiota profile of dogs with otitis externa. Vet Dermatol. 2021 Apr;32(2):158-e37. doi: 10.1111/vde.12930. Epub 2021 Jan 5. PMID: 33399257.
  • Puigdemont A, Furliani N, De Lucia M, Carrasco I, Ordeix L, Fondevila D, Ramio-Lluch L, Brazis P. Topical polyhydroxy acid-based treatment for autosomal recessive congenital ichthyosis in the golden retriever: a prospective study. Vet Dermatol. 2018 May 22. doi: 10.1111/vde.12654.
  • Ramió-Lluch L, Cerrato S, Brazis P, Rabanal RM, Fondevila D, Puigdemont A. Proof of concept of a new autologous skin substitute for the treatment of deep wounds in dogs. Vet J. 2017. 230:36-40. 
  • Balasch J, Ramió-Lluch L, Puigdemont A, Castro B, Bastida F.  Case report on the treatment of surgically debrided deep wounds with a new antioxidant wound dressing in two dogs. Adv Anim Vet Sci. 2016. 4: 389-393.
  • Cerrato S, Ramió-Lluch L, Brazís P, Fondevila D, Segarra S, Puigdemont A. Effects of sphingolipid extracts on the morphological structure and lipid profile in an in vitro model of canine skin. Vet J. 2016. June; 212:58-64.
  • Petrosino S, Schiano Moriello A, Cerrato S, Fusco M, Puigdemont A, De Petrocellis L, Di Marzo V. The anti-inflammatory mediator palmitoylethanolamide enhances the levels of 2-arachidonoyl-glycerol and potentiates its actions at trpv1 cation channels. Br J Pharmacol. 2015. Jan 19. doi: 10.1111/bph.13084.
  • Cerrato S, Ramió-Lluch L, Brazís P, Rabanal RM, Fondevila D, Puigdemont A. Development and characterization of equine skin-equivalent model. Vet Dermatol. 2014 Oct;25(5):475-e77
  • Cerrato S, Ramió-Lluch L, Fondevila D, Rodes D, Brazis P, Puigdemont A. Effects of essential oils and polyunsaturated fatty acids on canine skin equivalents: skin lipid assessment and morphological evaluation. J Vet Med 2013, Article ID 231526, 9 pages.
  • Cerrato S, Brazís P, Meana A, Fondevila D, Puigdemont A. In vitro development and characterization of canine epidermis on a porcine acellular dermal matrix. Vet J. 2012. Aug; 193(2):503-7.
  • Docampo MJ, Zanna G, Fondevila D, Cabrera J, López-Iglesias C, Carvalho A, Cerrato S, Ferrer L, Bassols A. Increased HAS2-driven hyaluronic acid synthesis in shar-pei dogs with hereditary cutaneous hyaluronosis (mucinosis). Vet Dermatol. 2011. Dec;22(6):535-45.
  • Serra M, Brazís P, Puigdemont A, Fondevila D, Romano V, Torre C, Ferrer L. Development and characterization of a canine skin equivalent. Exp Dermatol 2007. Feb; 16(2):135-4.
  • Torre C, Jeusette I, Serra M, Brazis P, Puigdemont A. Bovine colostrum increases proliferation of canine skin fibroblasts. J Nutr. 2006. Jul; 136(7 Suppl):2058S-2060S.
  • Reguera MJ, Rabanal R, Puigdemont A, Ferrer L. Canine mast cell tumors express Stem Cell Factor receptor (c-kit). Am J Dermatopathol. 2000. 22(1): 49-54.  


  • Foj R, Carrasco I, Clemente F, Scarampella F, Calvet A, Prats A, Vivancos S, Brazís P, Puigdemont A. Clinical efficacy of sublingual allergen-specific immunotherapy in 22 cats with atopic dermatitis. Vet Dermatol. 2021, Febr;32(1):67-e12. doi: 10.1111/vde.12926. Epub 2021 Jan 5. PMID: 33399258.
  • Moya R, Ramió-Lluch L, Parody N, Brazis P, Puigdemont A, Carnés J. Specific Dermatophagoides farinae extract for canine immunotherapy. Vet Dermatol. 2021 Apr;32(2):131-e29. doi: 10.1111/vde.12902. Epub 2020 Oct 28. PMID: 33112005.
  • Ramió‐Lluch L, Brazís P, Ferrer L and Puigdemont A. Allergen‐specific immunotherapy in dogs with atopic dermatitis: is owner compliance the main success‐limiting factor?. Veterinary Record 2020, 187: 493-493. https://doi.org/10.1136/vr.106024
  • Ráez-Bravo A, Granados JE, Serrano E, Dellamaria D, Casais R, Rossi L, Puigdemont A, Cano-Manuel FJ, Fandos P, Pérez JM, Espinosa J, Soriguer RC, Citterio C, López-Olvera JR. 2016. Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica. Parasit Vectors. 2016 Oct 21;9(1):558.
  • Moya R, Carnes J, Sinovas N, Ramió L, Brazís P, Puigdemont A. 2016. Immunoproteomic characterization of Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis. Vet Immunol Immunopathol. 2016; 180:1-8.
  • Lee KW, Blankenship K, Mckinney B, Kern G, Buch J, Greenwood J, Brazis P, Drouet L, Tambone C, Faas R, Weaver G. Proficiency monitoring of monoclonal antibody cocktail-based enzyme-linked immunosorbent assay for detection of allergen-specific immunoglobulin e in dogs. J Vet Diagn Invest. 2015. Jul;27(4):461-9.
  • Puigdemont A, Brazís P, Ordeix L, Dalmau A, Fuertes E, Olivar A, Pérez C, Ravera I. Efficacy of a new topical cyclosporine A formulation in the treatment of atopic dermatitis in dogs. Vet J. 2013 Aug;197(2):280-5.
  • Cerrato S, Brazis P, Della Valle Mf, Miolo A, Puigdemont A. Inhibitory effect of topical adelmidrol on antigen-induced skin wheal and mast cell behavior in a canine model of allergic dermatitis. BMC Vet Res. 2012. Nov 26; 8:230.
  • Lee Kw, Blankenship KD, Mccurry ZM, Mckinney B, Ruffner R, Esch Re, Tambone C, Faas R, Hermes D, Brazis P, Drouet L. Intra and inter-laboratory reproducibility of a monoclonal antibody cocktail based ELISA for detection of allergen specific IgE in dogs: proficiency monitoring of macELISA in six laboratories. Vet Immunol Immunopathol. 2012. Aug 15; 148(3-4):267-75.
  • Cerrato S, Brazis P, Della Valle Mf, Miolo A, Petrosino S, Di Marzo V, Puigdemont A. Effects of palmitoylethanolamide on the cutaneous allergic inflammatory response in Ascaris hypersensitive Beagle dogs. Vet J. 2012. Mar; 191(3):377-82.
  • Cerrato S, Brazis P, Della Valle Mf, Miolo A, Puigdemont A. Effects of palmitoylethanolamide on immunologically induced histamine, PGD2 and TNFalpha release from canine skin mast cells. Vet Immunol Immunopathol 2010. Jan 15; 133(1):9-15.
  • Goicoa A, Espino L, Rodriguez I, Puigdemont A, Brazis P, Rejas J. Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain. Acta Vet Hung. 2008. Jun; 56(2):163-71.
  • Brazis P, Serra M, Sellés A, Dethioux F, Biourge V, Puigdemont A. Evaluation of storage mite contamination of commercial dry dog food. Vet Dermatol. 2008. Aug; 19(4):209-14.
  • Puigdemont A, Brazís P, Serra M, Fondati A. Immunologic responses against hydrolyzed soy protein in dogs with experimentally induced soy hypersensitivity. Am J Vet Res. 2006. Mar; 67(3):484-8.
  • Brazís P, Barandica L, Garcia F, Clough G, Church MK, Puigdemont A. Dermal microdialysis in dog: in vivo assessment of the cyclosporin A effect on cutaneous histamine and prostaglandin release. Vet Dermatol. 2006. Jun;17 (3):169-74.
  • Serra M, Brazís P, Fondati A, Puigdemont A. Assessment of IgE binding to native and hydrolyzed soy protein in serum obtained from dogs with experimentally induced soy protein hypersensitivity. Am J Vet Res. 2006. Nov; 67(11):1895-900.
  • De Mora F, Puigdemont A, Torres R. The role of mast cells in atopy: what can we learn from canine models? A thorough review of the biology of mast cells in canine and human systems. Br J Dermatol. 2006. Dec; 155(6):1109-23.
  • Queralt M, Brazis P, Merlos M, Puigdemont A. In vitro effects of rupatadine on histamine and TNF-a release from skin mast cells and human mast cell line HMC-1. Inflamm Res. 2000. 49: 1-6.
  • Brazis P, Queralt M, De Mora F, Ferrer L, Puigdemont A. Stem cell factor enhances IgE-dependent histamine release and TNF-a production in canine cutaneous mast cells. Vet Immunol Immunopathol. 2000. 75: 97-108.
  • Brazis P, Torres R, Queralt M, De Mora F, Ferrer L, Puigdemont A. Evaluation of cell-surface IgE receptors on the canine mastocytoma cell line C2 maintained in continuous culture. Am J Vet. Res. 2002. 63(5):763-766.
  • Brazis P, De Mora, Ferrer L, Puigdemont A. IgE enhances FceRI expression and IgE-dependent TNF-alpha release from canine skin mast cells. Vet Immunol Immunopathol. 2002. 85:205-212.
  • Torres R, Castellarnau C, Ferrer L, Puigdemont A, Santamaria LF, De Mora F. Mast cells induce up-regulation of P-selectin and ICAM-1 on carotid endothelial cells in a new in vitro model of mast cell-to-endothelial cell communication. Immunol Cell Biol. 2002. 80: 170-177.


  • Cacheiro-Llaguno C, Parody N, Escutia MR, Carnés J. Role of Circulating Immune Complexes in the Pathogenesis of Canine Leishmaniasis: New Players in Vaccine Development. Microorganisms. 2021 Mar 30;9(4):712. doi: 10.3390/microorganisms9040712. PMID: 33808383; PMCID: PMC8066116.
  • Parody N, Cacheiro-Llaguno C, Osuna C, Renshaw-Calderón A, Alonso C, Carnés J. Circulating immune complexes levels correlate with the progression of canine leishmaniosis in naturally infected dogs. Vet Parasitol. 2019 Oct;274:108921. doi: 10.1016/j.vetpar.2019.108921. Epub 2019 Sep 8. PMID: 31536867.
  • Cacheiro-Llaguno C, Parody N, Renshaw-Calderón A, Osuna C, Alonso C, Carnés J. Vaccination with LetiFend® reduces circulating immune complexes in dogs experimentally infected with L. infantum. Vaccine. 2020 Jan 22;38(4):890-896. doi: 10.1016/j.vaccine.2019.10.078. Epub 2019 Nov 6. PMID: 31706810.
  • Fernández Cotrina J1, Iniesta V1, Monroy I1, Baz V1, Hugnet C2, Marañon F3, Fabra M4, Gómez-Nieto LC1, Alonso C5. A large-scale field randomized trial demonstrates safety and efficacy of the vaccine LetiFend® against canine leishmaniosis. Vaccine. 2018 Apr 5;36(15).
  • Pereira L, Abbehusen M, Teixeira C, Cunha J, Nascimento IP, Fukutani K, dos-Santos W, Barral A, Indiani de Oliveira C, Barral-Netto M, Soto M, Brodskyn CI. Vaccination with Leishmania infantum Acidic Ribosomal P0 but Not with Nucleosomal Histones Proteins Controls Leishmania infantum Infection in Hamsters. PLoS Negl Trop Dis. 2015 Feb; 9(2): e0003490.
  • Fernández-Cotrina J, Iniesta V, Belinchón-Lorenzo S, Muñoz-Madrid R, Serrano F, Parejo JC, Gómez-Gordo L, Soto M, Alonso C, Gómez-Nieto LC. Experimental model for reproduction of canine visceral leishmaniosis by Leishmania infantum. Vet Parasitol. 2013 Feb 18;192(1-3):118-28.
  • Carcelén JIniesta VFernández-Cotrina JSerrano FParejo JCCorraliza IGallardo-Soler AMarañón FSoto MAlonso CGómez-Nieto CThe chimerical multi-component Q protein from Leishmania in the absence of adjuvant protects dogs against an experimental Leishmania infantum infection. Vaccine. 2009 Oct 9;27(43):5964-73.
  • Iborra S, Parody N, Abánades DR, Bonay P, Prates D, Novais FO, Barral-Netto M, Alonso C, Soto M. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes Infect. 2008 Aug-Sep;10(10-11):1133-41.
  • Iniesta V, Corraliza I, Carcelén J, Gómez Gordo L, Fernández-Cotrina J, Parejo JC, Carrión J, Soto M, Alonso C, Gómez Nieto C. Leishmania major infection in susceptible and resistant mice elicit a differential humoral response against a total soluble fraction and defined recombinant antigens of the parasite. Parasitol Res. 2008 Apr;102(5):887-93.
  • Iborra S, Abánades DR, Parody N, Carrión J, Risueño RM, Pineda MA, Bonay P, Alonso C, Soto M. The immunodominant T helper 2 (Th2) response elicited in BALB/c mice by the Leishmania LiP2a and LiP2b acidic ribosomal proteins cannot be reverted by strong Th1 inducers. Clin Exp Immunol. 2007 Nov;150(2):375-85.
  • Iborra S, Carrión J, Anderson C, Alonso C, Sacks D, Soto M. Vaccination with the Leishmania infantum acidic ribosomal P0 protein plus CpG oligodeoxynucleotides induces protection against cutaneous leishmaniasis in C57BL/6 mice but does not prevent progressive disease in BALB/c mice. Infect Immun. 2005 Sep;73(9):5842-52.
  • Parody N, Soto M, Requena JM, Alonso C. Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection. Parasite Immunol. 2004 Jun-Jul;26(6-7):283-93.
  • Iborra S, Soto M, Carrión J, Nieto A, Fernández E, Alonso C, Requena JM. The Leishmania infantum acidic ribosomal protein P0 administered as a DNA vaccine confers protective immunity to Leishmania major infection in BALB/c mice. Infect Immun. 2003 Nov;71(11):6562-72.
  • Molano I, Alonso MG, Mirón C, Redondo E, Requena JM, Soto M, Nieto CG, Alonso C. A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum. Vet Immunol Immunopathol. 2003 Mar 20;92(1-2):1-13.
  • Soto M, Alonso C, Requena JM.The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-gamma) production by murine splenocytes. Clin Exp Immunol. 2000 Nov;122(2):212-8.
  • Soto M, Requena JM, Quijada L, Alonso C. Multicomponent chimeric antigen for serodiagnosis of canine visceral leishmaniasis. J Clin Microbiol. 1998 Jan;36(1):58-63.
  • Soto M, Requena JM, Quijada L, Alonso C. Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins. Clin Diagn Lab Immunol. 1996 Jul;3(4):387-91.
  • Soto M, Requena JM, Quijada L, Guzman F, Patarroyo ME, Alonso C. Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis. Immunol Lett. 1995 Nov;48(1):23-8.
  • Soto M, Requena JM, Quijada L, Angel SO, Gomez LC, Guzman F, Patarroyo ME, Alonso C. During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins. Clin Exp Immunol. 1995 May;100(2):246-52.
  • Soto M, Requena JM, Garcia M, Gómez LC, Navarrete I, Alonso C. Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania. J Biol Chem. 1993 Oct 15;268(29):21835-43.
  • Soto M, Requena JM, Alonso C. Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes. Mol Biochem Parasitol. 1993 Oct;61(2):265-74.